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2000 spr platform  (Biacore)

 
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    Biacore 2000 spr platform
    FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to <t>GyrA14.</t> D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.
    2000 Spr Platform, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2000 spr platform/product/Biacore
    Average 90 stars, based on 1 article reviews
    2000 spr platform - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries"

    Article Title: Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2022.101785

    FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to GyrA14. D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.
    Figure Legend Snippet: FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to GyrA14. D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.

    Techniques Used: Expressing, Binding Assay, Sequencing, Mutagenesis, Fluorescence, FACS



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    FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to <t>GyrA14.</t> D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.
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    FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to <t>GyrA14.</t> D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.
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    FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to <t>GyrA14.</t> D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.
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    https://www.bioz.com/result/spr sensor platform biacore 2000/product/Biacore
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    Image Search Results


    FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to GyrA14. D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries

    doi: 10.1016/j.jbc.2022.101785

    Figure Lengend Snippet: FACS of pooled libraries. A , Dot plot showing the expression and binding of pooled library. Two different gates, P30 and P31, were used to sort the populations showing the highest expression and binding. B , Pie chart of relative enrichment of mutants from each library after one round of sorting and deep sequencing in gates P30 and P31. C , Sorting of pooled V18G, V20G, and L36A library based on binding to GyrA14. D , Heat map of correlation coefficient between the binding MFI of mutants calculated from an individual library and the pooled library at different stringencies, where stringency is the minimum number of reads per mutant. FACS, fluorescence-activated cell sorting.

    Article Snippet: The aggregated protein was removed using centrifugation at 18000 g . The fraction of active protein remaining was measured by binding to GyrA14 on a Biacore 2000 SPR platform.

    Techniques: Expressing, Binding Assay, Sequencing, Mutagenesis, Fluorescence, FACS